Journal:

Article Title: Oligonucleotides form a duplex with non-helical properties on a positively charged surface

doi:

Figure Lengend Snippet: Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an ArrayWorx imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.

Article Snippet: The analysis of intensities from the Cy3 and Cy5 channels was done with ArrayWorx v.1.50 software (Applied Precision) from the original stitched images and bar graphs were generated in Microsoft Excel.

Techniques: Adsorption, Labeling, Concentration Assay, Standard Deviation

Journal:

Article Title: Oligonucleotides form a duplex with non-helical properties on a positively charged surface

doi:

Figure Lengend Snippet: Analysis of the washing eluate. (A) Patches of aminosilanized surface (3 mm2 each) were saturated with Cy5-labeled probes (red), followed by rinsing to remove excess probe as described in Materials and Methods. Cy3-labeled targets (green) were hybridized to these patches, rinsed to remove the unbound targets and washed in 2 µl of the washing buffer for 15 min (see Materials and Methods). A 0.2 µl aliquot was aspirated from the resulting washing buffer and spotted on a clean slide, which was subsequently imaged with Cy3 and Cy5 filter sets on an ArrayWorx Imager (Applied Precision). (B) The reverse experiments are also shown, where the targets and probes had been reversed. The bar graphs represent the normalized means and the standard deviations of the mean from four such 0.2 µl eluates.

Article Snippet: The analysis of intensities from the Cy3 and Cy5 channels was done with ArrayWorx v.1.50 software (Applied Precision) from the original stitched images and bar graphs were generated in Microsoft Excel.

Techniques: Labeling